07 膜タンパク質可溶化剤

Detergent Screening Set (first choice-II)


  • 製品コード
    DS06  Detergent Screening Set (first choice-II)
容 量 本体価格
1 set ¥15,000
1 set CHAPS,
Sodium cholate(purified),


 目的に合った界面活性剤を見つけるには数多くの界面活性剤を入手して評価する必要がある。本製品は、膜タンパク質の可溶化や結晶化によく使われている代表的なDetergent 5種類をセットにしたものである。



 マス肝細胞:ミクロソーム(電気泳動) 1)
 哺乳類の培養細胞:繊維芽細胞成長因子(抽出・精製) 2)
 ウシ肝細胞:T3 結合性タンパク質(抽出・精製) 3)
 NG108-15, Hybrid cell:Opiate recepters(抽出・精製) 4)
 リンパ球:5'-ヌクレオチターゼ(抽出・精製) 5)
 anti-GST antibody,etc.(非特異的吸着防止[SPR]) 6)
 Hepatic RHE(単離) 7)
 bacteriorhodospin(抽出) 8)
 マウス肝細胞:プロラクチンレセプター(抽出) 9)

 グラム陰性菌:tetracyclin cation/proton antiporter(抽出・精製) 10)
 ウシ心臓ミトコンドリア:ATP synthase(結晶化) 11)
 Paracoccus denitrificans:nitric oxide reductase BC complex(抽出・精製) 12)
 大腸菌:Na+/H+ antiporter(抽出・精製) 13)

 Trypanosoma cruzi:Trypanothione reductase(結晶化) 14)
 Rhodobacter sphaeroids:reaction center(結晶化) 15)
 Thermus thermophilus HB8:DNA excision repair enzyme UvrB(結晶化) 16)
 ヒト:17β-hydroxysteroid dehydrogenase (17β-HSD1)(結晶化) 17)
 大腸菌:SecE(抽出・精製) 18)
 大腸菌:ラクトース輸送担体(抽出) 19,20)
 HL-60,HL-60R:PKC(抽出) 21)
 ラット肺胞:SP-A, SP-D(抽出) 22)

 Trypanosoma cruzi:Trypanothione reductase(結晶化)14)


(1) 性状:

(2) n-Dodecyl-β-D-maltoside:    試験適合

(3) n-Octyl-β-D-glucoside:    試験適合

(4) Sodium cholate(purified):    試験適合

(5) MEGA-8:    試験適合



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14) R. L. Krauth-Siegel, C. Sticherling, I. Jost, C. T. Walsh, E. F. Pai, W. Kabsch and B. Lantwin, "Crystallization and Preliminary Crystallographic Analysis of Trypanothione Reductase from Trypanosoma Cruzi, the Causative Agent of Chagas' Disease", FEBS Lett., 1993, 317, 105.
15) J. P. Allen, "Crystallization of the Reaction Center from Rhodobacter Sphaeroides in a New Tetragonal form", Proteins, 1994, 20, 283.
16) A. Shibata, N. Nakagawa, M. Sugahara, R. Masui, R. Kato, S. Kuramitsu and K. Fukuyama, "Crystallization and Preliminary X-ray Diffraction Studies of a DNA Excision Repair Enzyme, UvrB, from Thermus thermophilus HB8", Acta Cryst., 1999, D55, 704.
17) S. X. Lin, D. W. Zhu, A. Azzi, R. L. Campbell, R. Breton, F. Labrie, D. Ghosh, V. Pletnev, W. L. Duax and W. Pangborn, "Studies on the Three-dimensional Structure of Estrogenic 17 beta-hydroxysteroid Dehydrogenase", J. Endocrinol., 1996, 150, S13.
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21) M. Nishikawa, F. Komada, Y. Uemura, H. Hidaka and S. Shirakawa, "Decreased Expression of Type II Protein Kinase C in HL-60 Variant Cells Resistant to Cell Differentiation by Phorbol Diester", Cancer Res., 1990, 50, 621.
22) J. R. Wright, D. F. Zlogar, J. C. Taylor, T. M. Zlogar and C. I. Restrepo, "Effects of Endotoxin on Surfactant Protein A and D Stimulation of NO Production by Alveolar Macrophages", Am. J. Physiol., 1999, 276, 650.


1.保存方法:冷蔵, 2.吸湿注意