07 膜タンパク質可溶化剤

Detergent Screening Set (first choice-II)

膜タンパク質可溶化剤

  • 製品コード
    DS06  Detergent Screening Set (first choice-II)
容 量 本体価格
1 set ¥15,000
キット内容
1 set CHAPS,
n-Dodecyl-β-D-maltoside,
n-Octyl-β-D-glucoside,
Sodium cholate(purified),
MEGA-8
以上5種類の各200mg包装

性質


界面活性剤は、膜タンパク質の可溶化や生化学試料溶液の安定化などに使用されている。しかし、どの界面活性剤が適しているのかは実際に使用してみないと分からないのが実情である。
 目的に合った界面活性剤を見つけるには数多くの界面活性剤を入手して評価する必要がある。本製品は、膜タンパク質の可溶化や結晶化によく使われている代表的なDetergent 5種類をセットにしたものである。
 どの界面活性剤が適しているかをまずはお試しいただきたい。

マニュアル

はじめての抗体標識プロトコル

死細胞数を測りたい(吸光測定)

Cytotoxicity LDH Assay Kit-WST を用いた細胞毒性試験の例を紹介

お役立ち情報

技術情報

応用例

・CHAPS
 マス肝細胞:ミクロソーム(電気泳動) 1)
 哺乳類の培養細胞:繊維芽細胞成長因子(抽出・精製) 2)
 ウシ肝細胞:T3 結合性タンパク質(抽出・精製) 3)
 NG108-15, Hybrid cell:Opiate recepters(抽出・精製) 4)
 リンパ球:5'-ヌクレオチターゼ(抽出・精製) 5)
 anti-GST antibody,etc.(非特異的吸着防止[SPR]) 6)
 Hepatic RHE(単離) 7)
 bacteriorhodospin(抽出) 8)
 マウス肝細胞:プロラクチンレセプター(抽出) 9)

n-Dodecyl-β-D-maltoside
 グラム陰性菌:tetracyclin cation/proton antiporter(抽出・精製) 10)
 ウシ心臓ミトコンドリア:ATP synthase(結晶化) 11)
 Paracoccus denitrificans:nitric oxide reductase BC complex(抽出・精製) 12)
 大腸菌:Na+/H+ antiporter(抽出・精製) 13)

n-Octyl-β-D-glucoside
 Trypanosoma cruzi:Trypanothione reductase(結晶化) 14)
 Rhodobacter sphaeroids:reaction center(結晶化) 15)
 Thermus thermophilus HB8:DNA excision repair enzyme UvrB(結晶化) 16)
 ヒト:17β-hydroxysteroid dehydrogenase (17β-HSD1)(結晶化) 17)
 大腸菌:SecE(抽出・精製) 18)
 大腸菌:ラクトース輸送担体(抽出) 19,20)
 HL-60,HL-60R:PKC(抽出) 21)
 ラット肺胞:SP-A, SP-D(抽出) 22)

・MEGA-8
 Trypanosoma cruzi:Trypanothione reductase(結晶化)14)

規格

(1) 性状:

(2) n-Dodecyl-β-D-maltoside:    試験適合

(3) n-Octyl-β-D-glucoside:    試験適合

(4) Sodium cholate(purified):    試験適合

(5) MEGA-8:    試験適合

参考文献

参考文献を表示する

1) G. H. Perdew, H. W. Schaup and D. P. Selivonchick, "The Use of a Zwitterionic Detergent in Two-dimensional Gel Electrophoresis of Trout Liver Microsomes", Anal. Biochem, 1983, 135, 453.
2) Y. Matuo, N. Nishi, Y. Muguruma, Y. Yoshitaka, Y. Masuda, K. Nishikawa and F. Wada, "The Usefulness of CHAPS as a Non-cytotoxic Stabilizing Agent in Purification of Growth Factors", Cytotechnology, 1998, 1, 309.
3) R. Horiuchi, K Yamauchi, H. hayashi, S. Koya, Y. Takeuchi, K. Kato, M. Kobayashi and H. Takikawa, "Purification and Characterization of 55-kDa Protein with 3,5,3'-Triiodo-L-thyronine-binding Activity and Protein Disulfide-isomerase Activity from Beef Liver Membrane", Eur. J. Biochem., 1989, 183, 529.
4) W. F. Simods, G. Koski, R. A. Streaty, L. M. Hjelmeland and W. A. Klee, "Solubilization of Avtive Opiate Receptors", Proc. Natl. Acad Sci.USA, 1980, 77, 4623.
5) M. T. Lehto and F. J. Sharom, "Release of the Glycosylphoaphatidylinositol-anchoroesd Enzyme Ecto-5'-Nucleotidase by Phopholipase C: Catalytic Activation and Modulation by the Lipid Bilayer", Biochem. J., 1998, 332, 101.
6) K. Andersson, M. Hamalainen and M. Malmqvist, "Identification and Optimaization of Regeneration Conditions for Affinity-based Biosensor Assays. A Multivariate Cocktail Approach", Anal. Chem., 1999, 71, 2475.
7) R. Scindler, R. Mentlein and W. Feldheim, "Purification and Characterization of Retinyl Ester Hydrolase as a Member of the Non-specific Carboxylesterase Supergene Family", Eur. J. Biochem., 1998, 251, 863.
8) J. Cladera, J. L. Rigaud, J. Villaverde and M. Dunach, "Liposome Solubilization and Membrane Protein Reconstitution Using Chaps and Chapso", Eur. J. Biochem., 1997, 243, 798.
9) D. S. Liscia, T. Alhadi and B. K. Vonderhaae, "Solubilization of Active Prolactin Receptors by a Nondenaturing Zwitterionic Detergent", J. Biol. Chem., 1982, 257, 9401.
10) C. Yin, M. L. A. Ramos, M. I. B. Walmsley, R. W. Taylor, A. R. Walmsley, S. B. levu and P. A. Bullough, "The Quarternary Molecular Architecture of TetA, A Secondary Tetracycline Transporter from Escherichia Coli", Molecular Microbiology, 2000, 38, 482 .
11) R. Lutter, M. Saraste, H. S. Walraven, M. J. Runswick, M. Finel, J. F. Deatherage and J. E. Walker, "F1F0-ATP Synthase from Bovine Heart Mitochndria: Development of the Purification of a Monodisperse Oligomycin-sensitivr ATPase", Biochem. J., 1993, 295, 799.
12) J. Hendriks, A. Warne, U. Gohlke, T. Haltia, C. Ludovici, M. Lubben and M. Saraste, "The Active Site of the Bacterial Nitric Cxide Reductase Is a Dinuclear Iron center", Biochemistry, 1998, 37, 13102.
13) K. A. Williams, U. Geldmacher-Kaufer, E. Padan, S. Schuldiner and W. Kuhlbrandt, "Projection Structure of NhaA, a Secondary Transporter from Escherichia Coli, at 4.0Å Resolution", EMBO J., 1999, 18, 3558.
14) R. L. Krauth-Siegel, C. Sticherling, I. Jost, C. T. Walsh, E. F. Pai, W. Kabsch and B. Lantwin, "Crystallization and Preliminary Crystallographic Analysis of Trypanothione Reductase from Trypanosoma Cruzi, the Causative Agent of Chagas' Disease", FEBS Lett., 1993, 317, 105.
15) J. P. Allen, "Crystallization of the Reaction Center from Rhodobacter Sphaeroides in a New Tetragonal form", Proteins, 1994, 20, 283.
16) A. Shibata, N. Nakagawa, M. Sugahara, R. Masui, R. Kato, S. Kuramitsu and K. Fukuyama, "Crystallization and Preliminary X-ray Diffraction Studies of a DNA Excision Repair Enzyme, UvrB, from Thermus thermophilus HB8", Acta Cryst., 1999, D55, 704.
17) S. X. Lin, D. W. Zhu, A. Azzi, R. L. Campbell, R. Breton, F. Labrie, D. Ghosh, V. Pletnev, W. L. Duax and W. Pangborn, "Studies on the Three-dimensional Structure of Estrogenic 17 beta-hydroxysteroid Dehydrogenase", J. Endocrinol., 1996, 150, S13.
18) H. Tokuda, J. Akimaru, S. Matsuyame, K. Nishiyama and S. Mizushima, "Purification of SecE and Reconctitution of SecE-dependent Protein Translocation Activity", Fed. Eur. Biochem. Soc., 1991, 279, 233.
19) 土屋友房,“膜タンパク質の可溶化と界面活性剤”, 化学と生物実験ライン5, 廣川書店, 1990.
20) M. J. Newman, D. L. Foster, T. H. Wilson and H. R. Kaback, "Purification and Reconstitution of Functional Lactose Carrier From Escherichia Coli", J. Biol. Chem., 1981, 256, 11804.
21) M. Nishikawa, F. Komada, Y. Uemura, H. Hidaka and S. Shirakawa, "Decreased Expression of Type II Protein Kinase C in HL-60 Variant Cells Resistant to Cell Differentiation by Phorbol Diester", Cancer Res., 1990, 50, 621.
22) J. R. Wright, D. F. Zlogar, J. C. Taylor, T. M. Zlogar and C. I. Restrepo, "Effects of Endotoxin on Surfactant Protein A and D Stimulation of NO Production by Alveolar Macrophages", Am. J. Physiol., 1999, 276, 650.

取扱条件

取扱条件
1.保存方法:冷蔵, 2.吸湿注意

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