Detergent Screening Set (for crystallization)
膜タンパク質可溶化剤 5種セット
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製品コードDS05 Detergent Screening Set (for crystallization)
| 容 量 | 本体価格 |
|---|---|
| 1 set | ￥19,000 |
性質
界面活性剤は膜タンパク質の結晶化に広く用いられている。本製品は、タンパク質の構造解析のための結晶化に用いられた実績のある5種類のDetergentを組み合わせたセットである。いずれの界面活性剤も高純度であり、得られたタンパク質結晶は高分解能で構造解析できる。
マニュアル
技術情報
結晶化への応用例
・n-Decyl-β-D-maltoside [code: D382]
ウシ心臓：cytochrome c oxidase 1)
グラム陰性菌：tetracyclin cation/proton antiporter 2)
・n-Dodecyl-β-D-maltoside [code: D316]
ウシ心臓ミトコンドリア：ATP synthase 3)
・n-Octyl-β-D-glucoside [code: O001]
Trypanosoma cruzi：Trypanothione reductase 4)
Rhodobacter sphaeroids：reaction center 5)
Thermus thermophilus HB8：DNA excision repair enzyme UvrB 6)
ヒト：17β-hydroxysteroid dehydrogenase (17β-HSD1) 7)
・MEGA-10 [code: M016]
ウシ心臓ミトコンドリア：ubiquinol-cytochrome c reductase 8)
参考文献
1) T. Tsukihara, H. Aoyama, E. Yamashita, T. Tomizaki, H. Yamaguchi, K. Shinzawa-Itoh, R. Nakashima, R. Yaono and S. Yoshikawa, "Structures of Metal sites of Oxidized Bovine Heart Cytochrome c Oxidase at 2.8 Å", Science, 1995, 269, 1069.
2) C. C. Yin, M. L. Aldema-Ramos, M. I. Borges-Walmsley, R. W. Taylor, A. R. Walmsley, S. B. Levy and P. A. Bullough, "The Quarternary Molecular Architecture of TetA, a Secondary Tetracycline Transporter from Escherichia coli", Mol. Microbiol., 2000, 38, 482.
3) R. Lutter, M. Saraste, H. S. Walraven, M. J. Runswick, M. Finel, J. F. Deatherage and J. E. Walker, " F1F0-ATP Synthase from Bovine Heart Mitochondria: Development of the Purification of a Monodisperse Oligomycin-sensitive ATPase", Biochem. J., 1993, 295, 799.
4) R. L. Krauth-Siegel, C. Sticherling, I. Jost, C. T. Walsh, E. F. Pai, W. Kabsch and C. B. Lantwin, "Crystallization and Preliminary Crystallographic Analysis of Trypanothione Reductase from Trypanosoma cruzi, the Causative Agent of Chagas' disease", FEBS Lett., 1993, 317, 105.
5) J. P. Allen, "Crystallization of the Reaction Center from Rhodobacter Sphaeroides in a New Tetragonal Form", Proteins, 1994, 20, 283.
6) A. Shibata, N. Nakagawa, M. Sugahara, R. Masui, R. Kato, S. Kuramitsu and K. Fukuyama, "Crystallization and Preliminary X-ray Diffraction Studies of a DNA Excision Repair Enzyme, UvrB, from Thermus Thermophilus HB8", Acta Crystallogr. D Biol. Crystallog, 1999, 55, 704.
7) S. X. Lin, D. W. Zhu, A. Azzi, R. L. Campbell, R. Breton, F. Labrie, D. Ghosh, V. Pletnev, W. L. Duax and W. Pangborn, "Studies on the Three-dimensional Sructure of Estrogenic 17 beta-Hydroxysteroid Dehydrogenase", J. Endocrinol., 1996, 150(2), 151.
8) W. H. Yue, Y. P. Zou, L. Yu and C. A. Yu, "Crystallization of Mitochondrial Ubiquinol-cytochrome c Reductase", Biochemistry, 1991, 30, 2303.
取扱条件
| n-Decyl-β-D-maltoside,： | 試験適合 |
|---|---|
| n-Dodecyl-β-D-maltoside： | 試験適合 |
| n-Octyl-β-D-glucoside,： | 試験適合 |
| n-Octyl-β-D-maltoside： | 試験適合 |
| MEGA-10： | 試験適合 |
| 1.保存方法：冷蔵, 2.吸湿注意 |
