Fig. 10

Transfection efficiencies of siRNA by amphiphilic compounds with different structures. A) Transfection efficiencies of siRNA by TMA-C2-Glu-C12, TMA-C4-Glu-C12, TMA-C6-Glu-C12, or TMA-C11-Glu-C12. CHO-EGFP cells were precultured in 24-well plates and transfected for 3.0 h with a mixture of EGFP siRNA and amphiphile (50 pmol/5.0 μl); transfection efficiencies were estimated as a decreased range of EGFP expression on a flow cytometry histogram at 24 h post-transfection. All data points are averaged from 2 replications on different days and expressed as the mean standard deviation. B) Transfection efficiencies of siRNA by TMA-C2-Glu-C14, TMA-C4-Glu-C14, or TMA-C6-Glu-C14. C) Transfection efficiencies of siRNA by TMA-C2-Asp-C12.