| Fig. 3 | Immunocytochemistry of mouse embryonic neural stem cells. Neural stem cells were cultured for 24 h in the DMEM/F12 medium with insulin, transferrin, chemically defined lipid concentrate, selenium, EGF, and FGF-basic in a fibronectin-coated 24-well plate. The cells were fixed with 2.0% paraformaldehyde solution and stained with anti-nestin antibody and Alexa Fluor 488-conjugated secondary antibody. Fluorescence images of nestin-positive neural cells: left, phase contrast image (×400); right, fluorescence image (×400). |
