Fig.10 Effect of proteinase K treatment on DNA releasing ability of the cytosolic fraction. The pDNA complex of KG6 was formed at a C/A of 1.0, and then the complex was mixed with the proteinase K digested cytosolic fraction, intact cytosolic fraction, or mock digested cytosolic fraction, for 3 h at 37��. The released DNA was electrophoresed in agarose gels (1% w/v) and stained with ethidium bromide.