Fig. 9 DNA release from gene carrier/DNA complexes mediated by addition of mouse liver cytosolic fraction. The DNA complexes of gene carriers were made at individual C/A ratios, and then mixed with the cytosolic fraction (Cytosol), homogenization buffer (Homo. buf.), or BSA, and incubated for 3 h at 37��. The released DNA was electrophoresed in agarose gels (1% w/v) and stained with ethidium bromide. O.C. and S.C. on the left of the gel indicate the position of the opencoiled and supercoiled forms of DNA.