Fig.2-B Effect of order of additions of the DnaK・J-GrpE set and ClpB on the recovery of LDH activity. The order of addition of chaperones are indicated. For all experiments, ATP (3 mmol/l) was added from the beginning of the 73 ℃ incubation. The LDH activities at 0 min (brank bars) and 60 min (black bars) after temperature shift to 55 ℃ were measured.