Fig.2-A Effect of the Dnak・J-GrpE set and ClpB on aggregation of lactate dehydrogenase (LDH). LDH,biotinylated for detection, was incubates in the presence of indicated components at 73 ℃ for 30 min.The solutions were centrifuged,and supernatant (sup) and precipitate (ppt) were analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate.

Electrophoresed proteins were blotted to the membrane, and biotinylated LDH was detected by alkaline phosphatase-conjugated streptavidin. KJ, E,and B represent DnaK・J comlex, GrpE and ClpB, respectively. All of the chaperones were isolated from Thermus thermophilus.