Fig.4　　Effects of acetylcholine (ACh) on membrane potentials (MPs) and [Ca²⁺]_i in native HEK and HEKBKαβ in a layer. (A) Effects of ACh on MPs measured with microelectrodes. (B) Effects of ACh on MPs (a, b) and [Ca²⁺]_i (c,d) were simultaneously measured with DiBAC₄(3) and Fura 2, respectively. Reference 16) with the permission from Japanese Pharmacological Society.