Diagnostic

Quantitative Determination of Target Element in Biological samples

1.Enzymatic Detection

(1) New Trinder’s Reagents (Oxidase system)

New Trinder‘s Reagents, which are highly water-soluble aniline derivatives, are applicable for the colorimetric determination of hydrogen peroxide. In the presence of a coupler such as 4-aminoantipyrine(4-AA), the Trinder‘s reagents are oxidized with peroxidase and two molecules of hydrogen peroxide and are developing the color to purple or blue.

 

(2) Tetrazolium Salts and Electron Mediators (Dehydrogenase system)

Tetrazolium salts have been developed to determine dehydrogenase activity which oxidizes its substrate and reduces its co-enzyme, NAD or NADP and produce NADH or NADPH. The colored formazan dyes are produced upon reduction of NADH in the presence of an electron mediator. To perform the homogenous assay in aqueous condition,  Water-Soluble Tetrazolium salts (WST) are widely applied for the diagnostic analysis and life science research due to much improved water-solubility than water insoluble tetrazolium salts, such like NBT and INT.

Detection Principle

Items

Principle (Oxidase System) New Trinder’s Reagents

Principle (Dehydrogenase System) Tetrazolium Salts and Electron Mediators

Cholesterol

Creatine

Glucose

Lactic acid

NH3 -

Pyruvate
Triglycerides

Uric acid -

2. Chelate Complexes Detection

(1) Other Chromogens for Clinical Chemical Assays

Dojindo offers wide range of reagents that form chelate complex with the target substance in biological sample. These reagents are applicable for quantitative determination of the target substance in the sample.

Items Reagent Principle
Calcium

Chlorophosphonazo-III

Ca2+ + Chlorophosphonazo-III → Ca2+ - Chlorophosphonazo-III complex 
Copper

3,5-DiBr-PAESA

Celuroplasmin – Cu2+ → Denaturation → Cu2+ → Reduction → Cu+  + 3,5-DiBr-PAESA → Cu+ - 3,5-DiBr-PAESA complex

Iron

Nitroso-PSAP

Transferrin – Fe3+ → Denaturation → Fe3+ → Reduction→ Fe2+  + Nitroso-PSAP → Fe2+ - Nitroso-PSAP complex

Magnesium

XB-I (Xylidyl blue-I)

Mg2+  + Xylidyl blue-I → Mg2+ - Xylidyl blue-I  complex

Urinary Total Protein

PR (Pyrogallol Red)

Pyrogallol Red  + Molybdate(VI) + Protein → PR – MO - Protein  complex

Zinc

5-Br-PAPS

Physiological Ligand – Zn2+ → Denaturation → Zn2+ + 5-Br-PAPS → Zn2+ - 5-Br-PAPS complex 

Nitro-PAPS

Zn2+  + Nitro-PAPS → Zn2+ –  Nitro-PAPS  complex


Oxidative Dyes (New Trinder’s Reagents )

Code#

Description

CAS#

OC01

ADOS

82692-96-4

OC02

ADPS

82611-88-9

OC04

ALPS

82611-85-6

OC06

DAOS

83777-30-4

OC08

HDAOS

82692-88-4

OC11

MAOS

82692-97-5

OC13

TOOS

82692-93-1

OC14

TOPS

40567-80-4

OC21

MADB

209518-16-1

OC22

TODB

1044537-70-3

OC46

EMSE

-


Reductive Dyes (Tetrazolium Salts )

Code#

Description

CAS#

W201

WST-1

150849-52-8

W202

WST-3

515111-36-1

W203

WST-4

178925-54-7

W204

WST-5

178925-55-8

W217

WST-9

-

I003

INT

146-68-9

M009

MTT

298-93-1

N011

Nitro-TB

298-83-9

T012

TB

1871-22-3


Oxidative Dyes (Others)

Code#

Description

CAS#

K257

KN-304

-

D006

DAB

7411-49-6

S302

SAT-3

679787-07-6

T022 

TMBZ

54827-17-7

T036

TMBZ-PS

102062-46-4,
102062-36-2(free acid)


Electron Mediators

Code#

Description

CAS#

M003

1-Methoxy PMS

65162-13-2

M470

1-Methoxy PES

133395-54-7


Chromogens for Chelate Complexes Detection

Code#

Description

CAS#

C010

Chlorophosphonazo-III

1914-99-4

D041

3,5-DiBr-PAESA

100743-65-5

N010

Nitroso-PSAP

80459-15-0

X001

XB-I

14936-97-1

P012

PR

32638-88-3

B026

5-Br-PAPS

81608-06-2(free acid)

N031

Nitro-PAPS

143205-66-7(as anhydride)


Substrate

Code#

Description

CAS#

G213

Glupa-C

63699-78-5

L005

L-Leucyl-p-nitroanilide

HCl

16010-98-3

N055

Disodium p-Nitrophenylphosphate

333338-18-4(hexahydrate)

Product Classification

Product Classification

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