02 Oxidative Stress

MDA Assay Kit

MDA Assay Kit

MDA(malondialdehyde) Assay Kit

  • Capable of measuring MDA in cells and tissues
  • Simple procedure
  • Product code
    M496  MDA Assay Kit
Unit size --
100 tests Please inquire distributors about price.

<Approximate number of uses> One plate of 96-well plate

Component
100 tests ・Lysis Buffer
・Dilution Buffer
・Standard
・Substrate
・Antioxidant
6.5 ml×1
10 ml×1
200 µl×1
58 mg×1
200 µl×1

Description

 Malondialdehyde (MDA) is one of the end products of lipid peroxides, the MDA concentration in cell or tissue samples can indicate lipid peroxidation which is important in the fields of oxidative stress and ferroptosis. MDA is also called reactive aldehyde and reacts with amino and thiol groups to cause protein denaturation and DNA damage, so it is also measured in various disease studies such as cancer and diabetes.
 Dojindo's MDA Assay Kit uses the TBARS method to detect MDA by measuring the fluorescence or absorbance of the adduct of MDA and thiobarbituric acid that develops color according to the amount of MDA. The kit also contains MDA standard solution, which can be used to prepare a standard curve and quantify the MDA concentration in the sample.

Manual

Technical info

  The fluorometric method can measure both cell samples and tissue samples, the colorimetric method can only measure tissue samples. Please choose the proper method according to the expected concentration of samples.
 

  Fluorometric Colorimetric Amount of Sample Limit of Detection
Cell OK × 1-3×107 cells 1-10 µmol
Tissue OK OK

Fluorometric: 10-30 mg

Colorimetric: 20-50 mg

Fluorometric: 1-10 µmol

Colorimetric: 1-50 µmol

 

Simple Procedures

  Other company's TBA method MDA assay kit requires a substrate weighing procedure. however, Dojindo's Kit doesn't need a weighing procedure, thus greatly reducing the measuring time and the variation of assay results due to weighing errors.

Applicaiton Data: MDA level in Ferroptosis induced Cells

 Using Dojindo's MDA Assay Kit measuring the changes of MDA level in erastin (an inhibitor of xCT, Cystine/Glutamate transporter) treated HepG2 cells, the result shows that the GSSH/GSH ratio is decreased and the MDA, intracellular Fe2+, ROS, Lipid peroxide level is increased.

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References

Open References

No. Sample Reference (Link)
1 Tissue
(mouse hippocampal formation)
K. Igarashi, H. Iwai, K. Tanaka, Y. Kuwahara, J. Kitanaka, N. Kitanaka, A. Kurimasa, K. Tomita, T. Sato, "Neuroprotective effect of oxytocin on cognitive dysfunction, DNA damage, and intracellular chloride disturbance in young mice after cranial irradiation", 2022, Biochem. Biophys. Res. Commun., doi:10.1016/j.bbrc.2022.04.099.

Q & A

Q

What is the principle of this kit?

A

This kit uses 2-thiobarbituric acid(TBS) as a substrate. By measuring the absorbance or fluorescence intensity of the TBA adduct formed by the reaction of MDA and TBA, and comparing with the standard curve, the MDA concentration in the sample can be simply quantified. 

Q

How many samples can be tested by this kit?

A

When testing the sample in triplicates, 24 samples can be tested by this kit.

Q

What is the storage condition of the prepared solution?

A

Substrate stock solution is stable for 2 months under -20℃.
Antioxidant PBS solution and Working solution is stable for 24 hours.

Q

Do I need to normalize the result by cell numbers?

A

If there is no change in cell number after drug stimulation, the normalization by cell number is not necessary.
If the number of cells changes, the normalized result by protein quantification is more accurate, in such case, the BCA method is recommended for protein quantification.

Q

What is the LOQ (Limit of Quantification) of this kit?

A

The limit of quantification of both the fluorometric method and the colorimetric method of MDA is 1 µmol/l.

Handling and storage condition

Handling and storage condition
1. Hazardous material Class 4, Petroleums No. 2, Hazardous material Class III, Petroleums No. 3, Hazardous material Class III, 2. Health and Safety Law, Chemical Substances Control Law, 3. Prevent from fire. 4. Store in a deep freeze at -20℃.
PRTR Policy Class 1 designated chemical substance
Danger / harmful
symbol mark
炎どくろ感嘆符腐食性健康有害性環境
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