siRNA 選択性蛍光プローブを用いた細胞内デリバリーイメージング解析

1) A. Fire, S. Xu, M. K. Montgomery, S. A. Kostas, S. E. Driver and C. C. Mello, “Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans”, Nature, 1998, 391, 806-810.

2) P. D. Zamore, T. Tuschl, P. A. Sharp and D. P. Bartel, “RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals”, Cell, 2000, 101, 25-33.

3) S. M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber and T. Tuschl, “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells”, Nature, 2001, 411, 494-498.

4) A. Gallas, C. Alexander, M. C. Davies, S. Puri and S. Allen, “Chemistry and formulations for siRNA therapeutics”, Chem. Soc., Rev., 2013, 42, 7983-7997.

5) E. Bernstein, A. A. Caudy, S. M. Hammond and G. J. Hannon, “Role for a bidentate ribonuclease in the initiation step of RNA interference”, Nature, 2001, 409, 363-366.

6) J.-B. Ma, K. Ye and D. J. Patel, “Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain”, Nature, 2004, 429, 318-322.

7) A. D. Judge, G. Bola, A. C. H. Lee and I. MacLachlan, “Design of noninflammatory synthetic siRNA mediating potent gene silencing in vivo”, Mol. Ther., 2006, 13, 494-505.

8) J. B. Bramsen, M. B. Laursen, A. F. Nielsen, T. B. Hansen, C. Bus, N. Langkjær, B. R. Babu, T. Høbjland, M. Abramov, A. Van Aerschot, D. Odadzic, R. Smicius, J. Haas, C. Andree, J. Barman, M. Wenska, P. Srivastava, C. Zhou, D. Honcharenko, S. Hess, E. Müller, G. V. Bobkov, S. N. Mikhailov, E. Fava, T. F. Meyer, J. Chattopadhyaya, M. Zerial, J. W. Engels, P. Herdewijn, J. Wengel and J. Kjems, “A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity”, Nucl. Acids Res., 2009, 37, 2867-2881.

9) A. Bouchie, “Companies in footrace to deliver RNAi”, Nat. Biotechnol., 2012, 30, 1154-1157.

10) M. V. Yezhelyev, L. Qi, R. M. O'Regan, S. Nie and X. Gao, “Proton-sponge coated quantum dots for siRNA delivery and intracellular imaging”, J. Am. Chem. Soc., 2008, 130, 9006-9012.

11) Y.-L. Chiu, A. Ali, C.-Y. Chu, H. Cao and T. M. Rana, “Visualizing a correlation between siRNA localization, cellular uptake, and RNAi in living cells”, Chem. Biol., 2004, 11, 1165-1175.

12) A. Järve, J. Müller, I.-H. Kim, K. Rohr, C. MacLean, G. Fricker, U. Massing, F. Eberle, A. Dalpke, R. Fischer, M. F. Trendelenburg and M. Helm, “Surveillance of siRNA integrity by FRET imaging”, Nucl. Acids Res., 2007, 35, e124.

13) A. S. Wahba, F. Azizi, G. F. Deleavey, C. Brown, F. Robert, M. Carrier, A. Kalota, A. M. Gewirtz, J. Pelletier, R. H. E. Hudson and M. J. Damha, “Phenylpyrrolocytosine as an unobtrusive base modification for monitoring activity and cellular trafficking of siRNA”, ACS Chem. Biol., 2011, 6, 912-919.

14) Y. Kamiya, A. Ito, H. Ito, M. Urushihara, J. Takai, T. Fujii, X. Liang, H. Kashida and H. Asanuma, “Selective labeling of mature RISC using siRNA carrying fluorophore-quencher pair”, Chem. Sci., 2013, 4, 4016-4021.

15) Y.-L. Chiu, and T. M. Rana, “RNAi in human cells: basic structural and functional features of small interfering RNA”, Mol. Cell, 2002, 10, 549-561.

16) T. Sato, Y. Sato, K. Iwai, S. Kuge, S. Nishizawa and N. Teramae, “Synthetic fluorescent probes capable of selective recognition of 3’-overhanging nucleotides for siRNA delivery imaging”, Chem. Commun., 2015, 51, 1421-1424.

17) T. Sato, Y. Sato, K. Iwai, S. Kuge, N. Teramae and S. Nishizawa, “Fluorescence imaging of siRNA delivery by peptide nucleic acid-based probe”, Anal. Sci., 2015, 31, 315-320.

18) C. A. Alabi, K. T. Love, G. Sahay, T. Stutzman, W. T. Young, R. Langer and D. G. Anderson, “FRET-labeled siRNA probes for tracking assembly and disassembly of siRNA nanocomplexes”, ACS Nano, 2012, 6, 6133-6141.

19) H. Hong, Y. Zhang and W. Cai, “In vivo imaging of RNA interference”, J. Nucl. Med., 2010, 51, 169-172.