Fig. 12 | Time courses of the absorbance change by the WST-1 assay. DL-Glyceraldehyde or glycolaldehyde (5 mM), Nƒ¿-acetyl-L-lysine (10 mM) and DTPA (50 ƒÊM) in 50 mM chelex-treated phosphate buffer (pH 7.0) were incubated for 2 days at 37Ž.The assay was performed in the absence (œ, £) and the presence (›, ¢) of SOD. |