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1) S. Avrameas,gCoupling of enzymes to protein with glutaraldehyde. Use of the conjugate for the detection of antigen and antibodiesh, Immunochemistry, 1969, 6, 43.

2) D. M. Boorsma and G. L. Kalsbeek,gA comparative study of horseradish peroxidase conjugates prepared with one-step and two-step methodh, J. Histochem. Cytochem., 1975, 23, 200.

3) D. M. Boorsma and J. G. Streefkerk,gPeroxidase-conjugate chromatography. Isolation of conjugate prepared with glutaraldehyde or periodate using polyacrylamide-agarose gelh, J. Histochem. Cytochem., 1976, 24, 481.

4) D. M. Boorsma, J. G. Streefkerk and N. Kors,gPreoxidase and fluorescein isothiocyanate as antibody marker. A quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate and a fluorescein conjugateh, J. Histochem. Cytochem., 1976, 24, 1017.

5) E. Engvall and P. Perlmann,gEnzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin Gh, Immunochemistry, 1971, 8, 871.

6) K. Kato, Y. Hamaguchi, H. Fukui and E. Ishikawa,gEnzyme-linked immunoassay I. Novel method of synthesis of the insulin-ƒÀ-D-galactosidase conjugate and its applicable for insulin assayh, J. Biochem., 1975, 78, 235.

7) P. K. Nakane and A. Kawaoi,gPeroxidase-labelled antibody. New method of conjugationh, J. Histochem. Cytochem., 1974, 22, 1084.

8) P. K. Nakane,gPreparation and standardization of enzyme-labelled conjugates.h, PP.81, in :R. M. Nakamura, W. R. Dito and E. S. Tucker, ‡V(eds.) :Immunoassey in the Clinical Laboratory, Alan R. Liss Inc., New York, 1979.

9) M. B. Wilson and P.K. Nakane,gResent developments in the periodate method of conjugating horseradish peroxidase(HRPO) to antibodiesh. pp.215, In : W.Knapp, K. Holuber and G. Wick(eds.) :

Immunofluorescence and related staining techniques, Elsevier/North-Holland Biomedical Press, Amsterdam, 1978.