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Fig. 10 Fluorometric activity assay of an enzyme entrapped in a supramolecular hydrogel 3, and semi-wet peptide/protein chip using 3. a) Fluorescence change by enzymatic hydrolysis. The arrow represents the direction of fluorescence spectral change. b) Time course of the fluorescence spectral change. c) Direct observation of fluorescence change with the naked eyes. Pep-1 after cleavage by LEP in the hydrogel (left), Pep-1 in hydrogel without LEP (right). d) Preparation scheme of the supramolecular peptide/protein array. e) Assay of LEP inhibitors using supramolecular hydrogel-based protein chip. Condition: [LEP] = 1.0 μM, [3] = 0.25 wt% in 50 mM Tris buffer (pH 8.5), [pep-1] = 40 μM). |
