Fig.5
5-dimentional analysis of Ca-dependent fluorescence signals by spectral confocal microscopy.

a, 3D images (XY, XZ and YZ) of Yellow Cameleon 3.6 (YC 3.6)-expressed HeLa cell before and after stimulation.

b, spectral changes in YC3.6 fluorescence upon Ca-dependent FRET between CFP and YFP. Data was courtesy from Cell Imaging Press, Nikon.

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