Fig.12 Anti-BG antibody titer of rheumatoid arthritis and ANCA-associated vasculitis patients

ELISA plate was coated with CSBG (25 mg/mL was used to coat plate). The sera of patients or healthy donors was added to the plate, and the plate-bound Ig was determined with peroxidase-conjugeted anti Human IgG+IgM. Enzyme activity was measured by the addition of TMB substrate. In collaboration with Dr. M. Yoshida in Tokyo Medical University Hachioji Medical Center.