Fig.1 Various methods for peptide-immobilization

(a) activated ester or epoxide modified support, (b) maleimide-modified support, (c) cyclopentadien- modified peptide is immobilized with Diels-Alder reaction, (d) immobilization through shiff-base using formyl-modified support and aminooxy-peptide, (e) peptide is immobilized through thiazoline-ring formation, (f) thioester-modified support and native chemical ligation