Chart 2 Procedure for the derivatization of urinary basic amino acids
| Urine (100-times diluted) | 100 μL | |
 |
50 mM Triethylamine | 10 μL |
| 1 mM PSE (DMSO) | 100 μL | |
Dilute 10 times with mobile phase |
||
| Reaction mixture | ||
| Apply onto HPLC (20 μL) | ||
HPLC conditions |
| Coumn: TSKgel Super ODS ( 100×4.6 mm I.D., 2μm, Tosoh) |
| Mobile phase: CH3CN-H2O-CH3COOH (55:45: 1, v/v) |
| Flow rate; 1.0 mL/min |
| Fluorescence detection: Ex 345 nm, Em 475 nm |
