Chart 1 Procedure for the excimer fluorescence derivatization of polyamines
| Sample solution* | 200 μL | |
 |
1M K2CO3 | 10 μL |
| 5 mM PSE in CH3CN | 200 μL | |
| Heat at 100℃ for 20 min | ||
| Reaction mixture | ||
| Inject into HPLC(20 μL) | ||
| *THF:DMSO:H2O=1:2:1(V/V) | ||
| Polyamines Structure | Abbreviation |
| Putrescine H2N(CH2)4 NH2 | Put |
| Cadaverine H2N(CH2)5 NH2 | Cad |
| Spermidine H2N(CH2)3 NH(CH2)4NH2 | Spd |
| Spermine H2N(CH2)3 NH(CH2)4NH(CH2) 3NH2 | Spm |
| HPLC conditions |
| Column; TSKgel Super Octyl |
| (100x4.6 mm I.D., 2 μm) |
| Mobile phase;50-80% CH3CN(0-20 min,v/v) |
| Flow rate;1.0 mL/min |
| Fluorescence measurement;Excimer fluorescence:Ex345 nm,Em475 nm. |
