| Chart 2 Determination of MethylgIyoxal in Mouse Blood | |
| Blood | 5μl |
| Water | 100μl |
| Ultrafiltrate through a Centricon 30 ultrafilter(1500g,20min) | |
| Filtrate(blood sample) | 50μl |
| 7.0mmol/l DMB solution(1.0mol/l b-mercaptoethanol,28mmol/l Na2O4) | 50μl |
| Warm at 60℃ for 40min | |
| Apply onto HPLC(10μl)
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| HPLC conditions | |
| Column:Radial-Pak cartridge C18(100X8mm i.d,;5μm) | |
| Mobile phase : MeOH-CH3CN-40mmol/l phosphate buffer(pH 7.0)(44:7:49,v/v) | |
| Flow-rate:1.0ml/min | |
| Fluorescence detection : Ex.355nm;Em.393nm | |
