Table 2
Number of complexation sites (n) and complexation constant (K) with CHP self-aggregates

Protein
Moleculer
weight
n
K / M-1
Insulin (Ins)
5,735
5(CHP-55-1.1)
ND
9.3}0.3(CHP-55-1.7)
(1.8}0.2)~106
11.3}0.3(CHP-55-3.4)
(1.5}0.1)~106
Cytochrome c(Cytc)
12,400
4.3}1.0(CHP-55-1.6)
(8.1}3.0)~103
Myoglobin (Myb)
17,800
1.9}0.3(CHP-55-1.6)
(1.9}0.8)~104
-Chymotrypsin (Chy)
25,000
2.0}0.2(CHP-55-1.6)
(1.9}0.3)~105
Bovine serum
66,000
1.1}0.1(CHP-55-1.1)
(22.3}3.2)~106
albumin (BSA)
0.9}0.03(CHP-55-1.7)
(8.1}1.3)~106
0.98}0.02(CHP-55-3.4)
(2.6}0.2)~106

ND : Not determined. CHP-55-1.1 : Mw 55000 pullulan, 1.1 cholesterol per 100 glucoses unit