リボソームによるタンパク質合成の１分子力学測定
Single molecule force measurement for protein synthesis on the ribosome

Figure 5
Rupture force distributions for ribosome complexes assembled on mRNAs containing (a-e) or lacking(f-i)the SD sequence.

All complexes were assembled in 5 mM Mg²⁺. (a, f) Ribosome-mRNA complex without tRNAs. No tethered beads were observed in the absence of the SD sequence(f),indicating that the complex was too unstable for a force measurement under these conditions.(b,g)Ribosome-mRNA complex carrying deacylated tRNA^fMet in the P site.(c, h)Ribosome-mRNA complex with tRNA^fMet in the P site and Phe-tRNA^Phe in the A site. (d,i) Ribosome-mRNA complex with tRNA^fMet in the P site and N-acetyl-Phe-tRNA^Phe in the A site.(e) Ribosome-mRNA complex after ribosome-catalyzed peptide bond formation. The complex was assembled with fMet-tRNA^fMet in the P site and Phe-tRNA^Phe in the A site, and incubated for 20 min to allow for peptidyl transfer.