Figure 4
Examples showing the behavior of the bead.
(a)The position of tethered beads fluctuates around the tether
center. Once the bead is trapped by the optical tweezers, its fluctuations become suppressed as indicated. As the bead starts to follow the stage movement, the force exerted on the complex increases. When the ribosome complex gets ruptured, the bead returns to the trapping center. (b)Control measurement for ribosomes covalently crosslinked to cysteine-reactive surface. No rupture events were observed within our measurement range.
(c) Control measurement for a biotinylated RNA oligonucleotide designed to mimic the extension in the 16S rRNA. The RNA oligonucleotide was attached to streptavidin-derivatized surface and hybridized with the DNA oligonucleotide-bead conjugate. No rupture events were observed. |
